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Kinesin-1 activity recorded in living cells with a precipitating dye

Authors :
Simona Angerani
Eric Lindberg
Nikolai Klena
Christopher K. E. Bleck
Charlotte Aumeier
Nicolas Winssinger
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

Monitoring the activity of the processive motor protein kinesin-1 in live cells is currently difficult. Here the authors report the fluorogenic small molecule QPD-OTf, a kinesin-1 substrate that causes activity-dependent dye precipitation.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.84c103ff473b4a30af353c47c941bdf3
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-021-21626-1