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Quantitative analysis by reversed-phase high-performance liquid chromatography and retinal neuroprotection after topical administration of moxonidine
- Source :
- International Journal of Ophthalmology, Vol 13, Iss 3, Pp 390-398 (2020)
- Publication Year :
- 2020
- Publisher :
- Press of International Journal of Ophthalmology (IJO PRESS), 2020.
-
Abstract
- "AIM: To determine moxonidine in aqueous humor and iris-ciliary body by reversed-phase high performance liquid chromatography (RP-HPLC), and to evaluate the retinal neuroprotective effect after topical administration with moxonidine in a high intraocular pressure (IOP) model. METHODS: The eyes of albino rabbits were administered topically and ipsilaterally with 0.2% moxonidine. A RP-HPLC method was employed for the identification and quantification of moxonidine between 2 and 480min, which presented in the aqueous humor and iris-ciliary body. Flash electroretinography (F-ERG) amplitude and superoxide dismutase (SOD) level were measured between day 1 and day 15 after topical administration with moxonidine in a rabbit model of high IOP. Histological and ultrastructural observation underwent to analyze the changes of retinal morphology, the inner retinal layers (IRL) thickness, and retinal ganglion cell (RGC) counting. RESULTS: Moxonidine was detectable between 2 and 480min after administration, and the peak concentration developed both in the two tissues at 30min, 0.51 µg/mL in aqueous humor and 1.03 µg/g in iris-ciliary body. In comparison to control, F-ERG b-wave amplitude in moxonidine eyes were significantly differences between day 3 and day 15 (P
Details
- Language :
- English
- ISSN :
- 22223959 and 22274898
- Volume :
- 13
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- International Journal of Ophthalmology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.84636e7da244e5ba9e948dcdcc6bad7
- Document Type :
- article
- Full Text :
- https://doi.org/10.18240/ijo.2020.03.04