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Engineered yeast genomes accurately assembled from pure and mixed samples

Authors :
Joseph H. Collins
Kevin W. Keating
Trent R. Jones
Shravani Balaji
Celeste B. Marsan
Marina Çomo
Zachary J. Newlon
Tom Mitchell
Bryan Bartley
Aaron Adler
Nicholas Roehner
Eric M. Young
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

The cost and complexity of whole genome sequencing limits its use in identifying and validating sequences used for genetic engineering and synthetic biology. Here the authors present Prymetime, an integrated workflow to sequence engineered strains and identify engineering in metagenomes.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.84252f6c5bb479cb7fcefe72ee9704b
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-021-21656-9