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Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus

Authors :
Tatiana S. Novikova
Evgeny A. Ermakov
Elena V. Kostina
Alexander N. Sinyakov
Alexey E. Sizikov
Georgy A. Nevinsky
Valentina N. Buneva
Source :
Current Issues in Molecular Biology, Vol 45, Iss 12, Pp 9887-9903 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Anti-DNA antibodies are known to be classical serological hallmarks of systemic lupus erythematosus (SLE). In addition to high-affinity antibodies, the autoantibody pool also contains natural catalytic anti-DNA antibodies that recognize and hydrolyze DNA. However, the specificity of such antibodies is uncertain. In addition, DNA binding to a surface such as the cell membrane, can also affect its recognition by antibodies. Here, we analyzed the hydrolysis of short oligodeoxyribonucleotides (ODNs) immobilized on the microarray surface and in solution by catalytic anti-DNA antibodies from SLE patients. It has been shown that IgG antibodies from SLE patients hydrolyze ODNs more effectively both in solution and on the surface, compared to IgG from healthy individuals. The data obtained indicate a more efficient hydrolysis of ODNs in solution than immobilized ODNs on the surface. In addition, differences in the specificity of recognition and hydrolysis of certain ODNs by anti-DNA antibodies were revealed, indicating the formation of autoantibodies to specific DNA motifs in SLE. The data obtained expand our understanding of the role of anti-DNA antibodies in SLE. Differences in the recognition and hydrolysis of surface-tethered and dissolved ODNs need to be considered in DNA microarray applications.

Details

Language :
English
ISSN :
45120617, 14673045, and 14673037
Volume :
45
Issue :
12
Database :
Directory of Open Access Journals
Journal :
Current Issues in Molecular Biology
Publication Type :
Academic Journal
Accession number :
edsdoj.83c7de25dc9e4570aaf20eede051c3ac
Document Type :
article
Full Text :
https://doi.org/10.3390/cimb45120617