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Lower Expression of SPRY4 Predicts a Poor Prognosis and Regulates Cell Proliferation in Colorectal Cancer

Authors :
Xiaoling Zhou
Sheng Xie
Chunluan Yuan
Li Jiang
Xiaoyan Huang
Liumei Li
Yan Chen
Lichuan Luo
Jianxuan Zhang
Daogang Wang
Lijian Liu
Wei Shi
Liang Han
Nong Tang
Yunxi Ji
Source :
Cellular Physiology and Biochemistry, Vol 40, Iss 6, Pp 1433-1442 (2016)
Publication Year :
2016
Publisher :
Cell Physiol Biochem Press GmbH & Co KG, 2016.

Abstract

Background/Aims: Colorectal cancer (CRC) is the third most common type of cancer worldwide. Sprouty proteins are modulators of mitogeninduced signal transduction processes and therefore can influence the process of cancerogenesis. The encoded protein of Sprouty homolog 4 (SPRY4) is associated with various human cancers. However, its biological role and clinical significance in CRC development and progression are unknown. Methods: The aim of this study was to evaluate the expression and biological role of SPRY4 in colorectal cancer. qRT-PCR was performed to investigate the expression of SPRY4 in tumor tissues and corresponding non tumor colorectal tissues from 70 patients. The effect of SPRY4 on proliferation was evaluated by MTT and colony formation assays. CRC cells transfected with SPRY4 were injected into nude mice to study the effect of SPRY4 on tumorigenesis in vivo. Results: The lower expression of SPRY4 was remarkably correlated with deep tumor invasion and advanced TNM stage. Multivariate analyses revealed that SPRY4 expression served as an independent predictor for overall survival. Using 5-aza treatment, we also observed that SPRY4 expression can be affected by DNA methylation. Further experiments revealed that overexpressed SPRY4 significantly inhibited CRC cell proliferation both in vitro and in vivo. Conclusion: Our study demonstrated that SPRY4 is involved in the development and progression of colorectal cancer by regulating cell proliferation and shows that SPRY4 may be a potential diagnostic and prognostic target in patients with colorectal cancer.

Details

Language :
English
ISSN :
10158987 and 14219778
Volume :
40
Issue :
6
Database :
Directory of Open Access Journals
Journal :
Cellular Physiology and Biochemistry
Publication Type :
Academic Journal
Accession number :
edsdoj.838e789de24dfb8c871c3162bd1555
Document Type :
article
Full Text :
https://doi.org/10.1159/000453195