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β-caryophyllene improves cerebral ischemia reperfusion injury in rats via Notch1/NF-κB signal axis
- Source :
- Di-san junyi daxue xuebao, Vol 43, Iss 3, Pp 218-225 (2021)
- Publication Year :
- 2021
- Publisher :
- Editorial Office of Journal of Third Military Medical University, 2021.
-
Abstract
- Objective To explore the protective effect of β-caryophyllene (BCP) on cerebral ischemia-reperfusion (I/R) rats and related mechanism. Methods SD rats were randomized into sham operation group, I/R group, and low-, medium- and high-dose BCP groups (102, 204 and 408 mg·kg-1), Jagged1 (Notch1 agonist, 50 mg·kg-1 in 3 h before I/R injury)+BCP group, and Jagged1 group. The treatments were given to the corresponding rats through intragastric infusion. In 7 d later, SD rat CIR model was established with suture-occlusion, with ischemia for 1.5 h followed by reperfusion for 48 h. The infarct volume and neurobehavioral score were measured; Western blotting was used to detect the cerebral protein levels of Notch1, NF-κB p65, NF-κB p-p65 and Hes1; HE staining was applied to observe the number of dead cells, and ELISA was employed to detect the changes of TNF-α and IL1-β contents in the ischemic hippocampus. Results BCP treatment could significantly improve the neurological deficit, reduce the infarct volume, down-regulate the protein levels of Notch1, NF-κB and Hes1 in the hippocampus (P < 0.05), reduce the releases of TNF-α and IL-1β (P < 0.05), and attenuate the death of hippocampal neuronal cells in the rats after cerebral I/R injury. Comparing the simple Jagged1 intervention group, Notch1 agonist, Jagged1, could antagonize the therapeutic effects of BCP. Conclusion BCP can attenuate cerebral I/R injury in rats, and exerts its protective effect probably through inhibiting Notch1/NF-κB signal axis to reduce the release of inflammatory factors.
Details
- Language :
- Chinese
- ISSN :
- 10005404
- Volume :
- 43
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Di-san junyi daxue xuebao
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.829be1908f144a79846fe6c877c3174c
- Document Type :
- article
- Full Text :
- https://doi.org/10.16016/j.1000-5404.202009067