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Pdx-1 or Pdx-1-VP16 protein transduction induces β-cell gene expression in liver-stem WB cells
- Source :
- BMC Research Notes, Vol 2, Iss 1, p 3 (2009)
- Publication Year :
- 2009
- Publisher :
- BMC, 2009.
-
Abstract
- Abstract Background Pancreatic duodenal homeobox-1 (Pdx-1) or Pdx-1-VP16 gene transfer has been shown to induce in vitro rat liver-stem WB cell conversion into pancreatic endocrine precursor cells. High glucose conditions were necessary for further differentiation into functional insulin-producing cells. Pdx-1 has the ability to permeate different cell types due to an inherent protein transduction domain (PTD). In this study, we evaluated liver-to-pancreas conversion of WB cells following Pdx-1 or Pdx-1-VP16 protein transduction. Findings WB cells were grown in high glucose medium containing Pdx-1 or Pdx-1-VP16 recombinant proteins for two weeks. β-like cell commitment was analysed by RT-PCR of pancreatic endocrine genes. We found that WB cells in high glucose culture spontaneously express pancreatic endocrine genes (Pdx-1, Ngn3, Nkx2.2, Kir6.2). Their further differentiation into β-like cells expressing genes related to endocrine pancreas development (Ngn3, NeuroD, Pax4, Nkx2.2, Nkx6.1, Pdx-1) and β-cell function (Glut-2, Kir6.2, insulin) was achieved only in the presence of Pdx-1(-VP16) protein. Conclusion These results demonstrate that Pdx-1(-VP16) protein transduction is instrumental for in vitro liver-to-pancreas conversion and is an alternative to gene therapy for β-cell engineering for diabetes cell therapy.
- Subjects :
- Medicine
Biology (General)
QH301-705.5
Science (General)
Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 17560500
- Volume :
- 2
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- BMC Research Notes
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.8231ba9854ba4b45856a3e3263dd8a5c
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/1756-0500-2-3