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House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection

Authors :
Malik Aydin
Ella A. Naumova
Friedrich Paulsen
Wenli Zhang
Felix Gopon
Christian Theis
Sören Lutz
Eric Ehrke-Schulz
Wolfgang H. Arnold
Stefan Wirth
Anja Ehrhardt
Source :
Viruses, Vol 12, Iss 10, p 1151 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Adenovirus (AdV) infections in the respiratory tract may cause asthma exacerbation and allergic predisposition, and the house dust mite (HDM) may aggravate virus-induced asthma exacerbations. However, the underlying mechanisms of whether and how AdV affects asthmatic patients remains unclear. To address this question, we investigated nasal epithelial cells (NAEPCs) derived from a pediatric exacerbation study cohort for experimental analyses. We analyzed twenty-one different green-fluorescent protein- and luciferase-tagged AdV types in submerged 2D and organotypic 3D cell culture models. Transduction experiments revealed robust transduction of AdV type 5 (AdV5) in NAEPCs, which was associated with an increased uptake of AdV5 in the presence of HDM. In healthy and asthmatic NAEPCs exposed to HDM before infection, we observed a time- and dose-dependent increase of AdV5 uptake associated with upregulation of entry receptors for AdV5. Furthermore, electron microscopic and histologic analyses of 3D cell cultures revealed an impairment of the respiratory cilia after HDM exposition. This ex vivo pilot study shows the impact of AdV infection and HDM exposition in a primary cell culture model for asthma.

Details

Language :
English
ISSN :
19994915
Volume :
12
Issue :
10
Database :
Directory of Open Access Journals
Journal :
Viruses
Publication Type :
Academic Journal
Accession number :
edsdoj.81ff8d344457437eacdd4211c7838403
Document Type :
article
Full Text :
https://doi.org/10.3390/v12101151