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Analysis of Serum Proteome after Treatment of Osteoporosis with Anabolic or Antiresorptive Drugs

Authors :
Alvaro del Real
Sergio Ciordia
Carolina Sañudo
Carmen Garcia-Ibarbia
Adriel Roa-Bautista
Javier G. Ocejo-Viñals
Fernando Corrales
Jose A. Riancho
Source :
Metabolites, Vol 12, Iss 5, p 399 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

The aim of the study was to explore new markers in serum proteome associated with the response to antiosteoporosis drugs, namely teriparatide and denosumab. We obtained serum samples from 14 patients with osteoporosis, both at baseline and after 6 months of treatment with teriparatide (n = 10) or denosumab (n = 4). Samples were analyzed by nanoliquid chromatography coupled to high-resolution mass spectrometry on a QTOF 5600 (SCIEX) apparatus. The spectrometry data were analyzed with Mascot against the UniProtKB base and then several quality-control filters were applied for the identification of peptides (false discovery rate, FDR q < 0.02) and their quantification (FDR q < 0.05). In the group treated with teriparatide, 28 proteins were identified with significant differences before and after treatment. A pathway analysis by using the Reactome database revealed significant enrichment in the Insulin Like Growth Factor 1 (IGF-I) (FDR q 4 × 10−2) and innate immune system (FDR q 2 × 10−3) pathways. Among patients treated with denosumab, we observed significant differences in the levels of 10 proteins, which were also enriched in the pathways related to the innate immune system (FDR q 3 × 10−2). These results suggest that the innate immune system may be involved in the response to antiosteoporosis drugs.

Details

Language :
English
ISSN :
22181989
Volume :
12
Issue :
5
Database :
Directory of Open Access Journals
Journal :
Metabolites
Publication Type :
Academic Journal
Accession number :
edsdoj.81a8b687b42d4dd28977cb66641d97e1
Document Type :
article
Full Text :
https://doi.org/10.3390/metabo12050399