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Incorporation of Superparamagnetic Magnetic–Fluorescent Iron Oxide Nanoparticles Increases Proliferation of Human Mesenchymal Stem Cells

Authors :
Willian Pinheiro Becker
Juliana Barbosa Torreão Dáu
Wanderson de Souza
Rosalia Mendez-Otero
Rosana Bizon Vieira Carias
Jasmin
Source :
Magnetochemistry, Vol 10, Iss 10, p 77 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Mesenchymal stem cells (MSCs) have significant therapeutic potential and their use requires in-depth studies to better understand their effects. Labeling cells with superparamagnetic iron oxide nanoparticles allows real-time monitoring of their location, migration, and fate post-transplantation. This study aimed to investigate the efficacy and cytotoxicity of magnetic–fluorescent nanoparticles in human adipose tissue-derived mesenchymal stem cells (hADSCs). The efficacy of Molday ION rhodamine B (MIRB) labeling in hADSCs was evaluated and their biocompatibility was assessed using various techniques and differentiation assays. Prussian blue and fluorescence staining confirmed that 100% of the cells were labeled with MIRB and this labeling persisted for at least 3 days. Transmission electron microscopy revealed the internalization and clustering of the nanoparticles on the outer surface of the cell membrane. The viability assay showed increased cell viability 3 days after nanoparticle exposure. Cell counts were higher in the MIRB-treated group compared to the control group at 3 and 5 days and an increased cell proliferation rate was observed at 3 days post-exposure. Adipogenic, osteogenic, and chondrogenic differentiation was successfully achieved in all groups, with MIRB-treated cells showing an enhanced differentiation rate into adipocytes and osteocytes. MIRB was efficiently internalized by hADSCs but induced changes in cellular behavior due to the increased cell proliferation rate.

Details

Language :
English
ISSN :
23127481
Volume :
10
Issue :
10
Database :
Directory of Open Access Journals
Journal :
Magnetochemistry
Publication Type :
Academic Journal
Accession number :
edsdoj.81881822a4d43aa86972d24b8259083
Document Type :
article
Full Text :
https://doi.org/10.3390/magnetochemistry10100077