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In-solution Y-chromosome capture-enrichment on ancient DNA libraries
- Source :
- BMC Genomics, Vol 19, Iss 1, Pp 1-16 (2018)
- Publication Year :
- 2018
- Publisher :
- BMC, 2018.
-
Abstract
- Abstract Background As most ancient biological samples have low levels of endogenous DNA, it is advantageous to enrich for specific genomic regions prior to sequencing. One approach—in-solution capture-enrichment—retrieves sequences of interest and reduces the fraction of microbial DNA. In this work, we implement a capture-enrichment approach targeting informative regions of the Y chromosome in six human archaeological remains excavated in the Caribbean and dated between 200 and 3000 years BP. We compare the recovery rate of Y-chromosome capture (YCC) alone, whole-genome capture followed by YCC (WGC + YCC) versus non-enriched (pre-capture) libraries. Results The six samples show different levels of initial endogenous content, with very low (
Details
- Language :
- English
- ISSN :
- 14712164
- Volume :
- 19
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- BMC Genomics
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.816d1d78e11d419083ab8853a8bf6c49
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/s12864-018-4945-x