Biochemical assessment of amniotic membrane extract for optimal application in regenerative medicine

Objective Amniotic membrane (AM) extract is a promising tool in regenerative medicine, which offers similar therapeutic effects as intact AM but with a simpler and safer approach. AM displays several subregional variances.Method The quantitative analysis of hepatocyte growth factor (HGF) and transforming growth factor-beta 1 (TGF-β1) in placental and reflected AM sections were assayed by enzyme-linked immunosorbent assay.Results Detection of high levels of HGF and TGF-β1 with variation in concentration between the placental and reflected portions of the same membrane.Conclusion Understanding AM subregional variations would optimize AM extract preparation for specific regenerative medicine therapies.