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Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria

Authors :
Alam García-Heredia
Amol Arunrao Pohane
Emily S Melzer
Caleb R Carr
Taylor J Fiolek
Sarah R Rundell
Hoong Chuin Lim
Jeffrey C Wagner
Yasu S Morita
Benjamin M Swarts
M Sloan Siegrist
Source :
eLife, Vol 7 (2018)
Publication Year :
2018
Publisher :
eLife Sciences Publications Ltd, 2018.

Abstract

Rod-shaped mycobacteria expand from their poles, yet d-amino acid probes label cell wall peptidoglycan in this genus at both the poles and sidewall. We sought to clarify the metabolic fates of these probes. Monopeptide incorporation was decreased by antibiotics that block peptidoglycan synthesis or l,d-transpeptidation and in an l,d-transpeptidase mutant. Dipeptides complemented defects in d-alanine synthesis or ligation and were present in lipid-linked peptidoglycan precursors. Characterizing probe uptake pathways allowed us to localize peptidoglycan metabolism with precision: monopeptide-marked l,d-transpeptidase remodeling and dipeptide-marked synthesis were coincident with mycomembrane metabolism at the poles, septum and sidewall. Fluorescent pencillin-marked d,d-transpeptidation around the cell perimeter further suggested that the mycobacterial sidewall is a site of cell wall assembly. While polar peptidoglycan synthesis was associated with cell elongation, sidewall synthesis responded to cell wall damage. Peptidoglycan editing along the sidewall may support cell wall robustness in pole-growing mycobacteria.

Details

Language :
English
ISSN :
2050084X
Volume :
7
Database :
Directory of Open Access Journals
Journal :
eLife
Publication Type :
Academic Journal
Accession number :
edsdoj.8126d86165d48daa68baa4773114a87
Document Type :
article
Full Text :
https://doi.org/10.7554/eLife.37243