Back to Search
Start Over
Functional Activity of Human Hepatoma Cells Transfected with Adenovirus-Mediated Hepatocyte Nuclear Factor (HNF)-4 Gene
- Source :
- Cell Transplantation, Vol 13 (2004)
- Publication Year :
- 2004
- Publisher :
- SAGE Publishing, 2004.
-
Abstract
- Fulminant hepatic failure (FHF) is still associated with high mortality despite recent advances in medical management. There is need of an effective and safe bioartificial liver (BAL) support to help keep patients with FHF alive until an organ becomes available for transplantation or the native liver recovers. The aim of this study was to establish highly functional liver cells by means of transfecting hepatocyte nuclear factor (HNF)-4 gene for the development of BAL. We constructed adenovirus vector carrying rat HNF-4 cDNA, and transfected to hepatoma-derived cell lines, HepG2 and HuH-7, to enforce expression of the exogenous HNF-4 gene. We analyzed expression of HNF-4, HNF-1, and liver-specific genes in cells infected by the adenovirus vector expressing HNF-4. Adenovirus-mediated HNF-4 gene transfer resulted in increases in expressions of HNF-4, HNF-1, and liver-specific genes such as apolipoproteins, α1-antitrypsin (α1-AT), phosphoenolpyruvate carboxy-kinase, cytochrome P450 families, and glutamine synthetase in transfected hepatoma cells. Cells overexpressing HNF-4 removed ammonia from medium supplemented with NH 4 Cl to a greater extent than control cells. These findings demonstrated that transfected cell lines restored differentiated gene expressions and liver-specific function by the overproduction of HNF-4. HNF-4-overexpressing hepatocyte cell lines are useful for bioreactor of BAL systems.
- Subjects :
- Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 09636897, 15553892, and 00000000
- Volume :
- 13
- Database :
- Directory of Open Access Journals
- Journal :
- Cell Transplantation
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.7ec384219e304e07ae8f3c7ec0b1db9a
- Document Type :
- article
- Full Text :
- https://doi.org/10.3727/000000004783983855