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Impact of different culture media on hairy roots growth of Valeriana officinalis L.

Authors :
Ali PAKDIN PARIZI
Mohammad FARSI
Ghorban-Ali NEMATZADEH
Amin MIRSHAMSI
Source :
Acta Agriculturae Slovenica, Vol 103, Iss 2 (2015)
Publication Year :
2015
Publisher :
University of Ljubljana Press (Založba Univerze v Ljubljani), 2015.

Abstract

Transformed hairy root cultures of Valeriana officinalis were established by infection with Agrobacterium rhizogenes strain ATCC 15834. To determine the effect of different media on the growth of V. officinalis hairy roots, MS, B5 media (1.0X and 0.5X strength), N6 medium and a modified MS medium without phytohormones were used. In addition, different NH4+ to NO3- ratios in MS medium were studied. The effects of these treatments were evaluated after 21 days of culture in relation to hairy root growth. B5 and ½ B5 media were the best basal media for hairy root growth. MS medium supplemented with a 20:20 ratio (mM) of NH4+ to NO3- displayed highest growth rates and biomass yield in hairy root cultures. The present study demonstrated that the composition of culture medium and the ratio of different nitrogen sources have significant impact on the growth of V. officinalis hairy roots.

Details

Language :
English, Slovenian
ISSN :
18541941
Volume :
103
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Acta Agriculturae Slovenica
Publication Type :
Academic Journal
Accession number :
edsdoj.7d95f79d491949359575d097a850d4df
Document Type :
article
Full Text :
https://doi.org/10.14720/aas.2014.103.2.14