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Docking sites inside Cas9 for adenine base editing diversification and RNA off-target elimination

Authors :
Shuo Li
Bo Yuan
Jixin Cao
Jingqi Chen
Jinlong Chen
Jiayi Qiu
Xing-Ming Zhao
Xiaolin Wang
Zilong Qiu
Tian-Lin Cheng
Source :
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Publication Year :
2020
Publisher :
Nature Portfolio, 2020.

Abstract

Current SpCas9 adenine base editors that minimise RNA off-target activities have constrained editing windows. Here the authors use domain insertion of TadA into Cas9 to narrow, expand or shift the editing window with RNA off-target minimization simultaneously

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
11
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.7d58167fcfff4dcdb6820d39f8718c52
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-020-19730-9