Palliative effect of dietary common sage leaves against toxic impacts of nonylphenol in Mirror carp (Cyprinus carpio var specularis): Growth, gene expression, immune-antioxidant status, and histopathological alterations

Nonylphenol (NP) is a common toxicant that constitutes a potential hazard to the aquatic system. This study assessed the protective role of Salvia officinalis leaves powder (SOLP) against NP toxicity. We addressed mortalities, growth retardation, hepato-renal dysfunction, immune-antioxidant suppression, histopathological degeneration, and gene down-regulation induced by NP-toxicity. And the potential activity of SOLP against these negative impacts in Mirror carp (Cyprinus carpio var specularis). Acute toxicity assay (static 96-h) was primarily performed to investigate the 96-h LC50 of NP in C. carpio and it was recorded at 1.01 mg/L reckoned by Finney probit analysis. Fish (N = 240) were assigned to 4 treatments for 2 months. The first (control) and the second (SOLP) groups were fed on basal diets that were enriched with 0.0 and 4 g/kg of diet SOLP without NP exposure. The third (NP) and fourth (SOLP+NP) groups were provided with the same diets plus exposed to 1/10 of the 96 h-LC50 of NP (0.1 mg/L). The outcomes revealed that NP induced higher mortalities (46), significantly (p