Food-based modification of LC-PUFA concentration in complementary food did not affect plasma vitamin E concentration in infants

Background: ω-3 Long-chain polyunsaturated fatty acids (ω-3 LC-PUFA) are essential for an optimal development of infants. An increased dietary intake of ω-3 PUFA may also be associated with a decrease in the vitamin E content in the blood of healthy infants because these fatty acids have more isolated double bonds compared to ω-6 PUFA and are therefore more susceptible to lipid peroxidation. Methods: The present analysis is part of a randomised, food-based trial examining the influence of a 4–6-month intervention with an increased content of ω-3 PUFA (sources: rapeseed oil or salmon) of complementary meals on vitamin E concentration in plasma. From the introduction of complementary feeding at the age of 4–6 months until the age of 10 months, healthy infants born at term received vegetable–potato–meat meals either with α-linolenic acid (ALA)-rich rapeseed oil (intervention group rapeseed oil, IG-R), salmon in combination with corn oil (intervention group fish, IG-F) to provide docosahexaenoic acid (DHA), or with linoleic acid-rich corn oil (control group, CG). Blood plasma was obtained at the 4th month ±14 days (age of infants at baseline) and at the 10th month ±14 days (age of infants after 6 months of intervention) and analysed using HPLC (vitamin E and malondialdehyde (MDA)) and GC (fatty acids). Vitamin E (tocopherol profile) and MDA were measured in plasma samples and fatty acids were determined in plasma glycerophospholipids. Results: Complete data on vitamin E and fatty acid concentrations were available for 155 participants. Compared to CG, IG-F and IG-R had higher weekly α-tocopherol equivalents (α-TE) intake (p