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Angiogenesis PET Tracer Uptake (68Ga-NODAGA-E[(cRGDyK)]2) in Induced Myocardial Infarction and Stromal Cell Treatment in Minipigs

Authors :
Thomas Rasmussen
Bjarke Follin
Jens Kastrup
Malene Brandt-Larsen
Jacob Madsen
Thomas Emil Christensen
Morten Juhl
Smadar Cohen
Karsten Pharao Hammelev
Christian Holdflod Møller
Jens Peter Goetze
Philip Hasbak
Andreas Kjær
Source :
Diagnostics, Vol 8, Iss 2, p 33 (2018)
Publication Year :
2018
Publisher :
MDPI AG, 2018.

Abstract

Angiogenesis is considered integral to the reparative process after ischemic injury. The αvβ3 integrin is a critical modulator of angiogenesis and highly expressed in activated endothelial cells. 68Ga-NODAGA-E[(cRGDyK)]2 (RGD) is a positron-emission-tomography (PET) ligand targeted towards αvβ3 integrin. The aim was to present data for the uptake of RGD and correlate it with histology and to further illustrate the differences in angiogenesis due to porcine adipose-derived mesenchymal stromal cell (pASC) or saline treatment in minipigs after induction of myocardial infarction (MI). Three minipigs were treated with direct intra-myocardial injection of pASCs and two minipigs with saline. MI was confirmed by 82Rubidium (82Rb) dipyridamole stress PET. Mean Standardized Uptake Values (SUVmean) of RGD were higher in the infarct compared to non-infarct area one week and one month after MI in both pASC-treated (SUVmean: 1.23 vs. 0.88 and 1.02 vs. 0.86, p < 0.05 for both) and non-pASC-treated minipigs (SUVmean: 1.44 vs. 1.07 and 1.26 vs. 1.04, p < 0.05 for both). However, there was no difference in RGD uptake, ejection fractions, coronary flow reserves or capillary density in histology between the two groups. In summary, indications of angiogenesis were present in the infarcted myocardium. However, no differences between pASC-treated and non-pASC-treated minipigs could be demonstrated.

Details

Language :
English
ISSN :
20754418
Volume :
8
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Diagnostics
Publication Type :
Academic Journal
Accession number :
edsdoj.7c8508f8e7ba492b8b6f70550f0a8725
Document Type :
article
Full Text :
https://doi.org/10.3390/diagnostics8020033