The impact of the yttrium oxide nano particles Y2O3 on the in vitro fertilization and in vitro culture media in a mouse model

Introduction: Oxidative stress has a critical role in affecting in vitro fertilization (IVF) outcomes. The yttrium oxide nanoparticle (Y2O3 NPs) known as free radicals’ scavenger. The reactive oxygen species (ROS) has an essential role in the pathological effect of oxidative stress. The study aimed to evaluate the Y2O3 NPs effect on the in vitro fertilization media and in vitro culture media in mice as a mammalian model. Methods: The NPs of Y2O3 tested in two different concentrations, 25 μg/ml, and 50 μg/ml, to examine nanoparticle effect on cleavage rate and blastula rate by exposing the gametes cell for 6 h during the fertilization and 5 days on culture media till embryos reach the blastula stage. The Y2O3 NPs cubic shape, and the sizes used to be in the range of 18 ± 5 nm as measured by transmission electron microscopy (TEM) and 11.14 nm as measured by X rays diffractions (XRD). The results showed that the rate of blastocyst was significantly higher in the in the treated by adding the 25.ug/ml Y2O3 NP and the control groups than the higher treated group with 50ug/ml NP, group by the ad of Y2O3 nanoparticles. While the cleavage rate was unaffected by the Y2O3 nanoparticles. In conclusion the treatment of the Y2O3 in the IVF and early embryo development have no side effects except in higher rate in the blastula stage.