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Beneficial effect of Indigo Naturalis on acute lung injury induced by influenza A virus

Authors :
Peng Tu
Rong Tian
Yan Lu
Yunyi Zhang
Haiyan Zhu
Lijun Ling
Hong Li
Daofeng Chen
Source :
Chinese Medicine, Vol 15, Iss 1, Pp 1-18 (2020)
Publication Year :
2020
Publisher :
BMC, 2020.

Abstract

Abstract Background Infections induced by influenza viruses, as well as coronavirus disease 19 (COVID-19) pandemic induced by severe acute respiratory coronavirus 2 (SARS-CoV-2) led to acute lung injury (ALI) and multi organ failure, during which traditional Chinese medicine (TCM) played an important role in treatment of the pandemic. The study aimed to investigate the effect of Indigo Naturalis on ALI induced by influenza A virus (IAV) in mice. Method The anti-influenza and anti-inflammatory properties of aqueous extract of Indigo Naturalis (INAE) were evaluated in vitro. BALB/c mice inoculated intranasally with IAV (H1N1) were treated intragastrically with INAE (40, 80 and 160 mg/kg/day) 2 h later for 4 or 7 days. Animal lifespan and mortality were recorded. Expression of high mobility group box-1 protein (HMGB-1) and toll-like receptor 4 (TLR4) were evaluated through immunohistological staining. Inflammatory cytokines were also monitored by ELISA. Result INAE inhibited virus replication on Madin-Darby canine kidney (MDCK) cells and decreased nitric oxide (NO) production from lipopolysaccharide (LPS)-stimulated peritoneal macrophages in vitro. The results showed that oral administration of 160 mg/kg of INAE significantly improved the lifespan (P

Details

Language :
English
ISSN :
17498546
Volume :
15
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Chinese Medicine
Publication Type :
Academic Journal
Accession number :
edsdoj.7ac607ae4ad340178c57770d5e2f7aa5
Document Type :
article
Full Text :
https://doi.org/10.1186/s13020-020-00415-w