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Cordycepin inhibits proliferation of human keratinocyte cell line HaCaT induced by TNF-α
Cordycepin inhibits proliferation of human keratinocyte cell line HaCaT induced by TNF-α
- Source :
- Jichu yixue yu linchuang, Vol 43, Iss 9, Pp 1390-1393 (2023)
- Publication Year :
- 2023
- Publisher :
- Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College., 2023.
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Abstract
- Objective To study the effect of cordycepin on proliferation, apoptosis and inflammatory reaction of human keratinocyte cell line HaCaT induced by TNF-α. Methods HaCaT cells were divided into control group, model group and intervention groups including low, middle, high dose (cordycepin 25 μmol/L, 50 μmol/L and 100 μmol/L, respectively). HaCaT cells were incubated with by 40 μg/L TNF-α in model group and cordycepin intervention group for 24 hours. Then, the cordycepin intervention groups were incubated with corresponding cordycepin for 24 hours. Cell proliferation was detected by CCK-8 method. Apoptosis was detected by flow cytometry. And mRNA expression of interleukin (IL)-8, IL-1β was detected by RT-qPCR. Results After 24 hours of cordycepin incubation, cell proliferation rate decreased (P<0.05), cell apoptosis rate increased(P<0.05)and mRNA expression of IL-8 and IL-1β decreased (P<0.05). Conclusions Cordycepin can inhibit TNF-α-induced proliferation and inflammatory reaction of HaCaT cells and promote apoptosis of these cells.
- Subjects :
- human keratinocyte
cordycepin
psoriasis
cell proliferation
inflammation
Medicine
Subjects
Details
- Language :
- Chinese
- ISSN :
- 10016325
- Volume :
- 43
- Issue :
- 9
- Database :
- Directory of Open Access Journals
- Journal :
- Jichu yixue yu linchuang
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.7a3493e03024565bb2a60c21a192931
- Document Type :
- article
- Full Text :
- https://doi.org/10.16352/j.issn.1001-6325.2023.09.1390