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Cordycepin inhibits proliferation of human keratinocyte cell line HaCaT induced by TNF-α

Cordycepin inhibits proliferation of human keratinocyte cell line HaCaT induced by TNF-α

Authors :
LIANG Huiyong, WEI Qiuhui, DING Ya, PENG Xiaoqing, YAO Jing, SU Qijian
Source :
Jichu yixue yu linchuang, Vol 43, Iss 9, Pp 1390-1393 (2023)
Publication Year :
2023
Publisher :
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College., 2023.

Abstract

Objective To study the effect of cordycepin on proliferation, apoptosis and inflammatory reaction of human keratinocyte cell line HaCaT induced by TNF-α. Methods HaCaT cells were divided into control group, model group and intervention groups including low, middle, high dose (cordycepin 25 μmol/L, 50 μmol/L and 100 μmol/L, respectively). HaCaT cells were incubated with by 40 μg/L TNF-α in model group and cordycepin intervention group for 24 hours. Then, the cordycepin intervention groups were incubated with corresponding cordycepin for 24 hours. Cell proliferation was detected by CCK-8 method. Apoptosis was detected by flow cytometry. And mRNA expression of interleukin (IL)-8, IL-1β was detected by RT-qPCR. Results After 24 hours of cordycepin incubation, cell proliferation rate decreased (P<0.05), cell apoptosis rate increased(P<0.05)and mRNA expression of IL-8 and IL-1β decreased (P<0.05). Conclusions Cordycepin can inhibit TNF-α-induced proliferation and inflammatory reaction of HaCaT cells and promote apoptosis of these cells.

Details

Language :
Chinese
ISSN :
10016325
Volume :
43
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Jichu yixue yu linchuang
Publication Type :
Academic Journal
Accession number :
edsdoj.7a3493e03024565bb2a60c21a192931
Document Type :
article
Full Text :
https://doi.org/10.16352/j.issn.1001-6325.2023.09.1390