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Epitope mapping of the monoclonal antibody IP5B11 used for detection of viral haemorrhagic septicaemia virus facilitated by genome sequencing of carpione novirhabdovirus
- Source :
- Veterinary Research, Vol 54, Iss 1, Pp 1-7 (2023)
- Publication Year :
- 2023
- Publisher :
- BMC, 2023.
-
Abstract
- Abstract The monoclonal antibody (mAb) IP5B11, which is used worldwide for the diagnosis of viral haemorrhagic septicaemia (VHS) in fish, reacts with all genotypes of VHS virus (VHSV). The mAb exceptionally also reacts with the carpione rhabdovirus (CarRV). Following next generation genome sequencing of CarRV and N protein sequence alignment including five kinds of fish novirhabdoviruses, the epitope recognized by mAb IP5B11 was identified. Dot blot analysis confirmed the epitope of mAb IP5B11 to be associated with the region N219 to N233 of the N protein of VHSV. Phylogenetic analysis identified CarRV as a new member of the fish novirhabdoviruses.
- Subjects :
- Epitope mapping
mAb IP5B11
VHSV
Carpione rhabdovirus
Veterinary medicine
SF600-1100
Subjects
Details
- Language :
- English
- ISSN :
- 12979716
- Volume :
- 54
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Veterinary Research
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.79dbce3f45714f088ca9f4cc191144fa
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/s13567-023-01166-w