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Enhanced UHPLC-MS/MS screening of selective androgen receptor modulators following urine hydrolysis
- Source :
- MethodsX, Vol 7, Iss , Pp 100926- (2020)
- Publication Year :
- 2020
- Publisher :
- Elsevier, 2020.
-
Abstract
- Selective androgen receptor modulators (SARMs) represent non-steroidal agents commonly abused in human and animal (i.e. equine, canine) sports, with potential for further misuse as growth promoting agents in livestock-based farming. As a direct response to the real and possible implications of illicit application in both sport as well as food production systems, this study incorporated enzymatic hydrolysis (β-glucuronidase/arylsulfatase) into a previously established protocol while maintaining the minimal volume (200 µL) of urine sample required to detect SARMs encompassing various pharmacophores in urine from a range of species (i.e. equine, bovine, human, canine and rodent). The newly presented semi-quantitative UHPLC-MS/MS-based assay is shown to be fit-for-purpose, being rapid and offering high-throughput, with validation findings fulfilling criteria stipulated within relevant doping and food control legislation. • CCβ values determined at 1 ng mL−1 for majority of analytes. • Deconjugation step included in the method led to significantly increased relative abundance of ostarine in analysed incurred urine samples demonstrating the requirement for hydrolysis to detect a total form of emerging SARMs. • Assay amenable for use within routine testing to ensure fair play in animal and human sports and that animal-derived food is free from contamination with SARM residues.
- Subjects :
- SARMs
Urine
Hydrolysis
UHPLC-MS/MS
Doping analysis
Food safety
Science
Subjects
Details
- Language :
- English
- ISSN :
- 22150161
- Volume :
- 7
- Issue :
- 100926-
- Database :
- Directory of Open Access Journals
- Journal :
- MethodsX
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.7895ee007e24894841daecd9b153b35
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.mex.2020.100926