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Karyotypic characterization of Hydromedusa tectifera (Testudines, Pleurodira) from the upper Iguaçu River in the Brazilian state of Paraná
- Source :
- Genetics and Molecular Biology, Vol 29, Iss 2, Pp 263-266 (2006)
- Publication Year :
- 2006
- Publisher :
- Sociedade Brasileira de Genética, 2006.
-
Abstract
- We present the karyotypic characterization of 26 specimens of the side-necked turtle Hydromedusa tectifera collected in the upper Iguaçu River, Paraná state, Brazil. The turtles were cytogenetically analyzed using Giemsa staining and other banding techniques (C, G, Ag-NOR and CMA3) as well as fluorescence in situ hybridization (FISH) with a rDNA 18S probe. All the specimens showed a diploid number of 58 composed of 22 macro and 36 microchromosomes. The Ag-NOR, CMA3 and FISH techniques permitted the identification and characterization of the chromosome pairs bearing nucleolus organizer regions (NORs), while G-banding facilitated a better recognition and pairing of macrochromosomes. These data agree with some information available in the literature and should be very useful for further cytotaxonomic and cytosystematic studies.
- Subjects :
- chromosomes
FISH
G-banding
pleurodira
turtle
Genetics
QH426-470
Subjects
Details
- Language :
- English
- ISSN :
- 14154757 and 16784685
- Volume :
- 29
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- Genetics and Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.7720d2946b6e4a6e91db7ba3ff987f17
- Document Type :
- article
- Full Text :
- https://doi.org/10.1590/S1415-47572006000200011