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Koboku An Extract of Magnolia Bark, Inhibits Leukotriene Synthesis in Rat Basophilic Leukemia-1 Cells
- Source :
- Allergology International, Vol 46, Iss 3, Pp 187-193 (1997)
- Publication Year :
- 1997
- Publisher :
- Elsevier, 1997.
-
Abstract
- To determine anti-allergic effects of Koboku, a Chinese herbal medicine, we investigated its inhibitory action on the production of cysteinyl leukotrienes (LT) and LTB4, which are important chemical mediators in the pathogenesis of allergic diseases. A231 87-stimulated synthesis of cysteinyl LT and LTB4 was measured by HPLC in the absence or presence of various concentrations of Koboku in rat basophilic leukemia-1 (RBL-1) cells. In a dose-dependent manner, Koboku inhibited synthesis of cysteinyl LT and LTB4 by up to 92.3 and 100%, respectively. Immunoglobulin E-mediated release of cysteinyl LT and LTB4, which was measured by specific radioimmunoassay (RIA) was also inhibited by Koboku in RBL-2H3 cells. Sites of inhibition of Koboku in the metabolic pathway of LT synthesis were phospholipase A2 (PLA2) and 5-lipoxygenase (5-LO), but not LTC4 synthase or LTA4 hydrolase. We conclude that the anti-allergic effect of Koboku may be attributable, at least in part, to the inhibition of LT synthesis.
Details
- Language :
- English
- ISSN :
- 13238930
- Volume :
- 46
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Allergology International
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.76df5ae0cbe946c4a6aaf503c11f5a5b
- Document Type :
- article
- Full Text :
- https://doi.org/10.2332/allergolint.46.187