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Effects of oxidative stress on liver, brain and spinal cord of rats using L-NAME and treated with hydroxyurea. A model of sickle cell complication
- Source :
- Acta Cirúrgica Brasileira, Vol 35, Iss 3 (2020)
- Publication Year :
- 2020
- Publisher :
- Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2020.
-
Abstract
- Abstract Purpose: To analyze the serum levels of nitric oxide and correlate them with the levels of thiobarbituric acid reactive substances (TBARS) in liver, brain and spinal cord of animals using L-NAME and treated with hydroxyurea. Methods: Eighteen male albino Wistar rats were divided into three groups. NG-nitro-L-arginine methyl ester (L-NAME) was intraperitoneally administered to induce oxidative stress. TBARS and plasma nitric oxide levels were analyzed in all groups. Histopathology of the liver and vascular tissue was performed. Results: Statistically significant differences were seen in liver, brain and spinal cord TBARS levels. Conclusions: Following the use of L-NAME, hepatic tissue increased the number of Kupffer cells as oxidative stress and inflammatory response increased. The use of L-NAME caused an increase in lipid peroxidation products and, consequently, in oxidative stress in animals. Hydroxyurea doses of 35 mg / kg / day reduced TBARS values in liver, brain and spinal cord.
Details
- Language :
- English
- ISSN :
- 01028650
- Volume :
- 35
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Acta Cirúrgica Brasileira
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.76d72e4eff4a4ce7a3dfae12f57cb020
- Document Type :
- article
- Full Text :
- https://doi.org/10.1590/s0102-865020200030000001