Phytochemical analysis of high value medicinal plant Valeriana jatamansi using LC-MS and it's in-vitro anti-proliferative screening

Background: Phytochemicals are group of compounds derived from plant resource and shown to have many pharmacological activities. Identification and quantification of chemical constituents in complex matrix of plant is a challenging task. LC-MS is the most prominent analytical technique for this purpose. Purpose: The aim of the present study is identification and quantification of bioactive compounds from Valeriana jatamansi using LC-MS and their in-vitro anti-proliferative screening. Materials and Methods: HPLC-ESI-QTOF-MS/MS separation for qualitative analysis was performed with the aid of an Agilent Poroshell 120 ECC18 column (2.7 μm, 50 mm × 4.6 mm). For quantitative analysis UPLC-ESI-QqQLIT−MS/MS separation was achieved on ACQUITY UPLC BEH™ C18 column (1.7 μm, 50 mm × 2.1 mm). The eluents were water with 0.1% formic acid (A) and acetonitrile (B). The anti-proliferative activity of samples of V. jatamansi on exponentially growing breast cancer cells was determined by the sulphorhodamine B (SRB) assay. Results: An efficient and sensitive LC-MS method was developed for metabolite profiling of five samples of V. jatamansi which resulted in the identification of forty-seven compounds by HPLC-ESI-QTOF-MS/MS. Nine bioactive compounds were quantified for the first time in leaves and roots of V. jatamansi using UPLC-ESI-QqQLIT−MS/MS. Quantitation method was also validated according to ICH guidelines. The correlation coefficient values (r2> 0.9873), LODs and LOQs for each analyte were < 15.7 and 47.6 ng/mL. The intra and inter-day precision variations (RSD) are