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Ricin Antibodies’ Neutralizing Capacity against Different Ricin Isoforms and Cultivars

Authors :
Maria Lucia Orsini Delgado
Arnaud Avril
Julie Prigent
Julie Dano
Audrey Rouaix
Sylvia Worbs
Brigitte G. Dorner
Clémence Rougeaux
François Becher
François Fenaille
Sandrine Livet
Hervé Volland
Jean-Nicolas Tournier
Stéphanie Simon
Source :
Toxins, Vol 13, Iss 2, p 100 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

Ricin, a highly toxic protein from Ricinus communis, is considered a potential biowarfare agent. Despite the many data available, no specific treatment has yet been approved. Due to their ability to provide immediate protection, antibodies (Abs) are an approach of choice. However, their high specificity might compromise their capacity to protect against the different ricin isoforms (D and E) found in the different cultivars. In previous work, we have shown the neutralizing potential of different Abs (43RCA-G1 (anti ricin A-chain) and RB34 and RB37 (anti ricin B-chain)) against ricin D. In this study, we evaluated their protective capacity against both ricin isoforms. We show that: (i) RB34 and RB37 recognize exclusively ricin D, whereas 43RCA-G1 recognizes both isoforms, (ii) their neutralizing capacity in vitro varies depending on the cultivar, and (iii) there is a synergistic effect when combining RB34 and 43RCA-G1. This effect is also demonstrated in vivo in a mouse model of intranasal intoxication with ricin D/E (1:1), where approximately 60% and 40% of mice treated 0 and 6 h after intoxication, respectively, are protected. Our results highlight the importance of evaluating the effectiveness of the Abs against different ricin isoforms to identify the treatment with the broadest spectrum neutralizing effect.

Details

Language :
English
ISSN :
20726651
Volume :
13
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Toxins
Publication Type :
Academic Journal
Accession number :
edsdoj.760dd99b883743a88707867304cec55c
Document Type :
article
Full Text :
https://doi.org/10.3390/toxins13020100