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Coronin-1 is phosphorylated at Thr-412 by protein kinase Cα in human phagocytic cells
- Source :
- Biochemistry and Biophysics Reports, Vol 27, Iss , Pp 101041- (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Coronin-1, a hematopoietic cell-specific actin-binding protein, is thought to be involved in the phagocytic process through its interaction with actin filaments. The dissociation of coronin-1 from phagosomes after its transient accumulation on the phagosome surface is associated with lysosomal fusion. We previously reported that 1) coronin-1 is phosphorylated by protein kinase C (PKC), 2) coronin-1 has two phosphorylation sites, Ser-2 and Thr-412, and 3) Thr-412 of coronin-1 is phosphorylated during phagocytosis. In this study, we examined which PKC isoform is responsible for the phosphorylation of coronin-1 at Thr-412 by using isotype-specific PKC inhibitors and small interfering RNAs (siRNAs). Thr-412 phosphorylation of coronin-1 was suppressed by Gö6976, an inhibitor of PKCα and PKCβI. This phosphorylation was attenuated by siRNA for PKCα, but not by siRNA for PKCβ. Furthermore, Thr-412 of coronin-1 was phosphorylated by recombinant PKCα in vitro, but not by recombinant PKCβ. We next examined the effects of Gö6976 on the intracellular distribution of coronin-1 in HL60 cells during phagocytosis. The confocal fluorescence microscopic observation showed that coronin-1 was not dissociated from phagosomes in Gö6976-treated cells. These results indicate that phosphorylation of coronin-1 at Thr-412 by PKCα regulates intracellular distribution during phagocytosis.
Details
- Language :
- English
- ISSN :
- 24055808
- Volume :
- 27
- Issue :
- 101041-
- Database :
- Directory of Open Access Journals
- Journal :
- Biochemistry and Biophysics Reports
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.75e3a510a054fb7aee433fe299c5a6d
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.bbrep.2021.101041