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Participation of miR165a in the Phytochrome Signal Transduction in Maize (Zea mays L.) Leaves under Changing Light Conditions

Authors :
Dmitry N. Fedorin
Alexander T. Eprintsev
Victoria O. Chuykova
Abir U. Igamberdiev
Source :
International Journal of Molecular Sciences, Vol 25, Iss 11, p 5733 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

The involvement of the microRNA miR165a in the light-dependent mechanisms of regulation of target genes in maize (Zea mays) has been studied. The light-induced change in the content of free miR165a was associated with its binding by the AGO10 protein and not with a change in the rate of its synthesis from the precursor. The use of knockout Arabidopsis plants for the phytochrome A and B genes demonstrated that the presence of an active form of phytochrome B causes an increase in the level of the RNA-induced silencing miR165a complex, which triggers the degradation of target mRNAs. The two fractions of vesicles from maize leaves, P40 and P100 that bind miR165a, were isolated by ultracentrifugation. The P40 fraction consisted of larger vesicles of the size >0.170 µm, while the P100 fraction vesicles were

Details

Language :
English
ISSN :
14220067 and 16616596
Volume :
25
Issue :
11
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.74c088c4390467f9be8abef33b6f4fd
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms25115733