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Optimization of Lutein Complex Nanoparticle Preparation Process and Analysis of Stability and Antioxidant Activity

Authors :
Xin ZHANG
Jingmeng SUN
Zhenzhen JIA
Shiyu ZHANG
Jiahui ZHANG
Weiyu ZHANG
Source :
Shipin gongye ke-ji, Vol 45, Iss 16, Pp 102-113 (2024)
Publication Year :
2024
Publisher :
The editorial department of Science and Technology of Food Industry, 2024.

Abstract

To improve the solubility and stability of lutein (LUT), this study utilized the electrostatic self-assembly method to encapsulate LUT within food-grade protein nanoparticles and composite nanoparticles derived from soybean protein isolates (SPI) and fucoidin (FUD). The impact of the SPI/FUD mass ratio on the formation of ternary composite nanoparticles, known as lutein-soybean protein isolate-fucoidin nanoparticles (LUT-SPI-FUD NPs), was examined. The preparation process underwent optimization through single-factor experiments and the Box-Behnken response surface methodology. Various characterization techniques, including ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, differential scanning calorimetry and X-ray diffraction were employed to delve into the mechanisms governing the formation of these two types of nanoparticles to compare with their solubility, stability, and antioxidant properties. The results showed that LUT-SPI-FUD NPs with SPI/FUD mass ratio of 4:1 had an absolute value of zeta potential greater than 30 mV, a polydispersity index, and a smaller particle size. Under these conditions, the most favorable formulation and process parameters were as follows: A LUT/SPI mass ratio of 1:7.587, SPI mass concentration of 0.83 mg/mL, and a stirring time of 1.493 hours. Characterization analysis verified the ideal particle size and more concentrated distribution of both LUT-SPI NPs and LUT-SPI-FUD NPs, and the binding forces were the interplay of hydrogen bonds, hydrophobic interactions, and electrostatic forces. Solubility, stability, and antioxidant assessments indicated that the conversion of LUT into LUT-SPI NPs and LUT-SPI-FUD NPs substantially increased its solubility in purified water by 350.74 and 432.42 times, respectively. Moreover, both formulations very significant (P

Details

Language :
Chinese
ISSN :
10020306
Volume :
45
Issue :
16
Database :
Directory of Open Access Journals
Journal :
Shipin gongye ke-ji
Publication Type :
Academic Journal
Accession number :
edsdoj.74512a0ca9564cdb940b72aa63b0fd0e
Document Type :
article
Full Text :
https://doi.org/10.13386/j.issn1002-0306.2023100069