Back to Search
Start Over
Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.
- Source :
- Brazilian Archives of Biology and Technology, Vol 57, Iss 5, Pp 636-643 (2014)
- Publication Year :
- 2014
- Publisher :
- Instituto de Tecnologia do Paraná (Tecpar), 2014.
-
Abstract
- In the present study, the effects of two CaCl2.2H2O levels (440 and 1320 mg L-1) and two subcultures were evaluated on in vitro shoots of Lavandula angustifolia cv. Provence Blue. Ca2+ content of the apical, middle and basal portion of shoots was determined. Increasing CaCl2.2H2O level in the culture medium increased tissue Ca2+ content and decreased hyperhydricity. Shoot-tip necrosis also decreased with 1320 mg L-1 CaCl2.2H2O, but it did not occur in the second subculture. The middle and basal portion had higher Ca2+ content than apical portion. In non-hyperhydric tissues, there were smaller and more juxtaposed cells. Scanning electron microscopy of the leaves demonstrated that trichomes from in vitro leaf surface occurred in smaller quantities.
Details
- Language :
- English
- ISSN :
- 16784324 and 15168913
- Volume :
- 57
- Issue :
- 5
- Database :
- Directory of Open Access Journals
- Journal :
- Brazilian Archives of Biology and Technology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.73981295d1a248548a1f071bac086213
- Document Type :
- article
- Full Text :
- https://doi.org/10.1590/S1516-8913201402165