Back to Search Start Over

Traceability Research on Dendrobium devonianum Based on SWATHtoMRM

Authors :
Tao Lin
Xinglian Chen
Lijuan Du
Jing Wang
Zhengxu Hu
Long Cheng
Zhenhuan Liu
Hongcheng Liu
Source :
Foods, Vol 12, Iss 19, p 3608 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

SWATHtoMRM technology was used in this experiment to further identify and trace the sources of Dendrobium devonianum and Dendrobium officinale produced in the same area using TOF and MS-MRM. After the conversion of the R package of SWATHtoMRM, 191 MRM pairs of positive ions and 96 pairs of negative ions were obtained. Dendrobium devonianum and Dendrobium officinale can be separated very well using the PCA and PLS-DA analysis of MRM ion pairs; this shows that there are obvious differences in chemical composition between Dendrobium devonianum and Dendrobium officinale, which clearly proves that the pseudotargeted metabolomics method based on SWATHtoMRM can be used for traceability identification research. A total of 146 characteristic compounds were obtained, with 20 characteristic compounds in Dendrobium devonianum. The enrichment pathways of the characteristic compounds were mainly concentrated in lipids and atherosclerosis, chagas disease, fluid shear stress and atherosclerosis, proteoglycans in cancer, the IL-17 signaling pathway, the sphingolipid signaling pathway, diabetic cardiomyopathy, arginine and proline metabolism, etc., among which the lipid and atherosclerosis pathways were more enriched, and 11 characteristic compounds affected the expression levels of IL-1, TNFα, CD36, IL-1β, etc. These can be used as a reference for research on variety improvement and active substance accumulation in Dendrobium devonianum and Dendrobium officinale.

Details

Language :
English
ISSN :
23048158
Volume :
12
Issue :
19
Database :
Directory of Open Access Journals
Journal :
Foods
Publication Type :
Academic Journal
Accession number :
edsdoj.7385e592d39a4ff9a6f4113bd5ac38be
Document Type :
article
Full Text :
https://doi.org/10.3390/foods12193608