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Pyrazinoic acid-Poly(malic acid) biodegradable nanoconjugate for efficient intracellular delivery
- Source :
- Precision Nanomedicine, Vol 2, Iss 3 (2019)
- Publication Year :
- 2019
- Publisher :
- Andover House Inc., 2019.
-
Abstract
- Tuberculosis is an infectious disease, affecting mostly lungs, that is still considered a health global problem as it causes millions of deaths worldwide. Current treatment is effective, however, associated with severe adverse effects due to the high doses of each anti-tuberculosis drug daily administrated by oral therapy. For the first time, a pyrazinoic acid biodegradable nanoconjugate was synthesized and developed for pulmonary administration in an attempt to reduce the administered doses by achieving a high drug payload and controlled release at the target site. The conjugate was synthesized by coupling pyrazinoic acid on carboxylic groups of poly(malic acid), which is a biodegradable and biocompatible polymer, and posteriorly self-assembled into nanoconjugates. Characterization confirmed the formation of nanometric, spherical and negatively charged pyrazinoic acid nanoconjugate (NC-PA). NC-PA was stable for 60 days at 4 and 37 ºC and able to deliver PA in a sustained release manner over time. On macrophages, they exhibited no cell toxicity for a wide range of concentrations (from 1 to 100 µg/mL), demonstrating the safety of NC-PA. In addition, the nanoconjugate was efficiently taken up by RAW 264.7 cells over 6 hours reaching a maximum value after 3h of incubation. In conclusion, innovative nanoconjugates are a promising alternative to deliver drugs directly to the lungs and contributing to improving tuberculosis therapy. [READ ARTICLE](https://precisionnanomedicine.com/article/9660)
- Subjects :
- Medicine
Medical technology
R855-855.5
Subjects
Details
- Language :
- English
- ISSN :
- 26399431
- Volume :
- 2
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Precision Nanomedicine
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.732e3e64999b460483c02ef4c40a6450
- Document Type :
- article
- Full Text :
- https://doi.org/10.33218/prnano2(3).190523.1