Back to Search
Start Over
Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement
- Source :
- Engineering in Life Sciences, Vol 23, Iss 2, Pp n/a-n/a (2023)
- Publication Year :
- 2023
- Publisher :
- Wiley-VCH, 2023.
-
Abstract
- Abstract Clustering enzymes in the same metabolic pathway is a natural strategy to enhance productivity. Synthetic protein, RNA and DNA scaffolds have been designed to artificially cluster multiple enzymes in the cell, which require complex construction processes and possess limited slots for target enzymes. We utilized the Escherichia coli inner cell membrane as a native scaffold to cluster four fatty acid synthases (FAS) and achieved to improve the efficiency of fatty acid synthesis in vivo. The construction strategy is as simple as fusing target enzymes to the N‐terminus or C‐terminus of the membrane anchor protein (Lgt), and the number of anchored enzymes is not restricted. This novel device not only presents a similar efficiency in clustering multiple enzymes to that of other artificial scaffolds but also promotes the product secretion, driving the entire metabolic flux forward and further increasing the gross yield compared with that in a cytoplasmic scaffold system.
Details
- Language :
- English
- ISSN :
- 16182863 and 16180240
- Volume :
- 23
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- Engineering in Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.73122a247e574bf59403a6f1b40e784d
- Document Type :
- article
- Full Text :
- https://doi.org/10.1002/elsc.202200034