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Programmable molecular circuit discriminates multidrug-resistant bacteria
- Source :
- Materials Today Bio, Vol 16, Iss , Pp 100379- (2022)
- Publication Year :
- 2022
- Publisher :
- Elsevier, 2022.
-
Abstract
- Recognizing multidrug-resistant (MDR) bacteria with high accuracy and precision from clinical samples has long been a difficulty. For reliable detection of MDR bacteria, we investigated a programmable molecular circuit called the Background-free isothermal circuital kit (BRICK). The BRICK method provides a near-zero background signal by integrating four inherent modules equivalent to the conversion, amplification, separation, and reading modules. Interference elimination is largely owing to a molybdenum disulfide nanosheets-based fluorescence nanoswitch and non-specific suppression mediated by molecular inhibitors. In less than 70 min, an accurate distinction of various MDR bacteria was achieved without bacterial lysis. The BRICK technique detected 6.73 CFU/mL of methicillin-resistant Staphylococcus aureus in clinical samples in a proof-of-concept trial. By simply reprogramming the sequence panel, such a high signal-to-noise characteristic has been proven in the four other superbugs. The proposed BRICK method can provide a universal platform for infection surveillance and environmental management thanks to its superior programmability.
Details
- Language :
- English
- ISSN :
- 25900064
- Volume :
- 16
- Issue :
- 100379-
- Database :
- Directory of Open Access Journals
- Journal :
- Materials Today Bio
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.723490f5806d43e2a92980f571e8d2ee
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.mtbio.2022.100379