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Temporal Stability of Ciliary Beating Post Nasal Brushing, Modulated by Storage Temperature

Authors :
Noemie Bricmont
Romane Bonhiver
Lionel Benchimol
Bruno Louis
Jean-François Papon
Justine Monseur
Anne-Françoise Donneau
Catherine Moermans
Florence Schleich
Doriane Calmès
Anne-Lise Poirrier
Renaud Louis
Marie-Christine Seghaye
Céline Kempeneers
Source :
Diagnostics, Vol 13, Iss 18, p 2974 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Primary ciliary dyskinesia is a heterogeneous, inherited motile ciliopathy in which respiratory cilia beat abnormally, and some ultrastructural ciliary defects and specific genetic mutations have been associated with particular ciliary beating alterations. Ciliary beating can be evaluated using digital high-speed videomicroscopy (DHSV). However, normal reference values, essential to assess ciliary beating in patients referred for a PCD diagnostic, vary between centres, as minor variations in protocols might influence ciliary beating. Consequently, establishment of normal values is essential for each PCD diagnostic centre. We aimed to evaluate whether delay after sampling, and temperature for conservation of respiratory ciliated samples, might modify assessments of ciliary beating. In total, 37 healthy nasal brushing samples of respiratory ciliated epithelia were collected. Video sequences were recorded at 37 °C immediately using DHSV. Then, the samples were divided and conserved at 4 °C or at room temperature (RT). Ciliated beating edges were then recorded at 37 °C, at 3 h and at 9 h post sampling. In six samples, recordings were continued up to 72 h after sampling. Ciliary beating was assessed manually by ciliary beat frequency (CBFM) and ciliary beat pattern (CBP). A semi-automatic software was used for quantitative analysis. Both CBF and CBP evaluated manually and by a semi-automated method were stable 9 h after sampling. CBFM was higher when evaluated using samples stored at RT than at 4 °C. CBP and the semi-automated evaluation of ciliary beating were not affected by storage temperature. When establishing normal references values, ciliary beating can be evaluated at 37 °C up to 9 h after nasal brushing, but the storage temperature modifies ciliary beating and needs to be controlled.

Details

Language :
English
ISSN :
20754418
Volume :
13
Issue :
18
Database :
Directory of Open Access Journals
Journal :
Diagnostics
Publication Type :
Academic Journal
Accession number :
edsdoj.71e40134e8924e4982b44b22de49368a
Document Type :
article
Full Text :
https://doi.org/10.3390/diagnostics13182974