Rapid and simultaneous determination of 7 kinds of fishery anesthetics and 2 kinds of sedatives in freshwater fish by dispersive solid phase extraction liquid chromatography-mass spectrometry

ObjectiveA simple and rapid method for the simultaneous determination of 7 kinds of fishery anesthetics and 2 kinds of sedatives in freshwater fish was developed using liquid chromatography tandem mass spectrometry （LC-MS/MS）.MethodsThe fish sample was adsorbed with C18 and PSA after extracted with acetonitrile， then centrifuged and diluted with water， detected in the positive / negative ion mode. Quantitative analysis was carried out by isotope internal standard method with matrix calibration curve.ResultsUnder the optimized conditions， good linearities for 7 kinds of fishery anesthetics and 2 kinds of sedatives were obtained in the range of 0.5-50 μg/L （MS-222 was 0.05-5.0 μg/L） with the correlation coefficients（r） higher than 0.999. The limits of detection were 0.02-0.4 μg/kg （S/N=3）. Recoveries at three spiked levels ranged from 87.0% to 117.3%. And the relative standard deviations was 2.5%-8.2%.ConclusionThis method was simple， sensitive and selective， which could be applied to the rapid qualitative and quantitative analysis of the 7 kinds of fishery anesthetics and 2 kinds of sedatives in freshwater fish.