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Development of a duplex SYBR Green I-based quantitative real-time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses

Authors :
Su Lin
Shao Wang
Xiaoxia Cheng
Shifeng Xiao
Xiuqin Chen
Shilong Chen
Shaoying Chen
Fusong Yu
Source :
Virology Journal, Vol 16, Iss 1, Pp 1-6 (2019)
Publication Year :
2019
Publisher :
BMC, 2019.

Abstract

Abstract Background Waterfowl parvoviruses, including goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), can cause seriously diseases in geese and ducks. Developing a fast and precise diagnosis assay for these two parvoviruses is particularly important. Results A duplex SYBR Green I-based quantitative real-time PCR assay was developed for the simultaneous detection and differentiation of GPV and MDPV. The assay yielded melting curves with specific single peak (Tm = 87.3 ± 0.26 °C or Tm = 85.4 ± 0.23 °C) when GPV or MDPV was evaluated, respectively. When both parvoviruses were assessed in one reaction, melting curves with specific double peaks were yielded. Conclusion This duplex quantitative RT-PCR can be used to rapid identify of GPV and MDPV in field cases and artificial trials, which make it a powerful tool for diagnosing, preventing and controlling waterfowl parvovirus infections.

Details

Language :
English
ISSN :
1743422X
Volume :
16
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Virology Journal
Publication Type :
Academic Journal
Accession number :
edsdoj.715c15650ede40189f2a1f1f896504c9
Document Type :
article
Full Text :
https://doi.org/10.1186/s12985-018-1111-7