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Core-Shell-Shell Upconversion Nanomaterials Applying for Simultaneous Immunofluorescent Detection of Fenpropathrin and Procymidone

Authors :
Yang Song
Jingyi Jin
Liuling Hu
Bingqian Hu
Mengyao Wang
Lilong Guo
Xiyan Lv
Source :
Foods, Vol 12, Iss 18, p 3445 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

This study synthesized the NaGdF4@NaGdF4: Yb, Tm@NaGdF4: Yb, Nd upconversion nanoparticles (UCNPs), combined with another three-layer structure NaYF4@NaYF4: Yb, Er@NaYF4 UCNPs, with a core-shell-shell structure, effectively suppressing fluorescence quenching and significantly improving upconversion luminescence efficiency. Two types of modified UCNPs were coupled with antibodies against fenpropathrin and procymidone to form signal probes, and magnetic nanoparticles were coupled with antigens of fenpropathrin and procymidone to form capture probes. A rapid and sensitive fluorescence immunoassay for the simultaneous detection of fenpropathrin and procymidone was established based on the principle of specific binding of antigen and antibody and magnetic separation technology. Under the optimal competitive reaction conditions, different concentrations of fenpropathrin and procymidone standards were added to collect the capture probe-signal probe complex. The fluorescence values at 542 nm and 802 nm were measured using 980 nm excitation luminescence. The results showed that the detection limits of fenpropathrin and procymidone were 0.114 µg/kg and 0.082 µg/kg, respectively, with sensitivities of 8.15 µg/kg and 7.98 µg/kg, and they were applied to the detection of fenpropathrin and procymidone in tomatoes, cucumbers, and cabbage. The average recovery rates were 86.5~100.2% and 85.61~102.43%, respectively, with coefficients of variation less than 10%. The results showed good consistency with the detection results of high-performance liquid chromatography, proving that this method has good accuracy and is suitable for the rapid detection of fenpropathrin and procymidone in food.

Details

Language :
English
ISSN :
23048158
Volume :
12
Issue :
18
Database :
Directory of Open Access Journals
Journal :
Foods
Publication Type :
Academic Journal
Accession number :
edsdoj.711bf4fdb0f404d82fca72c566111c9
Document Type :
article
Full Text :
https://doi.org/10.3390/foods12183445