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Epidemiological and clinical features of Leishmania (Viannia) braziliensis American cutaneous and mucocutaneous leishmaniasis in the State of Espírito Santo, Brazil

Authors :
A Falqueto
PA Sessa
AL Ferreira
VP Vieira
CB Santos
JBM Varejão
E Cupolillo
R Porrozzi
LE Carvalho-Paes
G Grimaldi Jr
Source :
Memorias do Instituto Oswaldo Cruz, Vol 98, Iss 8, Pp 1003-1010 (2003)
Publication Year :
2003
Publisher :
Fundação Oswaldo Cruz (FIOCRUZ), 2003.

Abstract

Between 1985 and 2000, epidemiological surveys of the American tegumentary leishmaniasis (ATL) were carried out in several rural and urban communities in Espírito Santo, Brazil. A total of 100 stocks of Leishmania (comprising isolates from both human and canine hosts with ATL) were identified by two methods of molecular characterization, using specific monoclonal antibodies and multilocus enzyme electrophoresis. Parasite isolates from 19 municipalities were found to belong to the same zymodeme and serodeme type as of the Leishmania (Viannia) braziliensis reference strain. In contrast, our genotyping studies have shown intra-specific variation among these parasites (comparisons of the variability of the internal transcribed spacers between the small and large subunits of the rRNA genes of the 22 stocks studiedrevealed at least 11 genotypes). Two main clusters of L. (V.) braziliensis genotypes were observed, representing parasites collected from different endemic regions in the state, where transmission reflects distinct eco-epidemiological features. Infection with this pathogen was associated with the characteristic disease forms, but neither the clinical outcome nor the response to treatment could be related to the genetic polymorphism of the isolates, as defined by using the proposed methodology.

Details

Language :
English
ISSN :
00740276 and 16788060
Volume :
98
Issue :
8
Database :
Directory of Open Access Journals
Journal :
Memorias do Instituto Oswaldo Cruz
Publication Type :
Academic Journal
Accession number :
edsdoj.70c45c82a88e44d4b0a4fba7c97eb0af
Document Type :
article
Full Text :
https://doi.org/10.1590/S0074-02762003000800004