Effect of growth regulators and explant origin on in vitro propagation of Ceratonia siliqua L. via cuttings

The present work was undertaken to develop a basic and simple protocol for micropropagation of Ceratonia siliqua. Axillaries bud sprouting and shoot development were stimulated on MS supplemented with BAP (0.5 mg.l-1), IBA (0.1 mg.l-1) and GA3 (0.5 mg.l-1), shoot multiplication was obtained on MS supplemented with BAP (2 mg.l-1) and rooting of microshoots was achieved on MS supplemented with IBA (2 mg.l-1) and charcoal (2 mg.l-1). The best results were obtained with herbaceous explants taken from juvenile trees. Significant differences in proliferation, multiplication and rooting due to the type and origin of explant and to the concentration of growth regulators were found.