Back to Search Start Over

An Easy-to-Use Arrayed Brain–Heart Chip

Authors :
Xiyao Peng
Lei Wu
Qiushi Li
Yuqing Ge
Tiegang Xu
Jianlong Zhao
Source :
Biosensors, Vol 14, Iss 11, p 517 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Multi-organ chips are effective at emulating human tissue and organ functions and at replicating the interactions among tissues and organs. An arrayed brain–heart chip was introduced whose configuration comprises open culture chambers and closed biomimetic vascular channels distributed in a horizontal pattern, separated from each other by an endothelial barrier based on fibrin matrix. A 300 μm-high and 13.2 mm-long endothelial barrier surrounded each organoid culture chamber, thereby satisfying the material transport requirements. Numerical simulations were used to analyze the construction process of fibrin barriers in order to optimize the structural design and experimental manipulation, which exhibited a high degree of correlation with experiment results. In each interconnective unit, a cerebral organoid, a cardiac organoid, and endothelial cells were co-cultured stably for a minimum of one week. The permeability of the endothelial barrier and recirculating perfusion enabled cross talk between cerebral organoids and cardiac organoids, as well as between organoids and endothelial cells. This was corroborated by the presence of cardiac troponin I (cTnI) in the cerebral organoid culture chamber and the observation of cerebral organoid and endothelial cells invading the fibrin matrix after one week of co-culture. The arrayed chip was simple to manipulate, clearly visible under a microscope, and compatible with automated pipetting devices, and therefore had significant potential for application.

Details

Language :
English
ISSN :
20796374
Volume :
14
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Biosensors
Publication Type :
Academic Journal
Accession number :
edsdoj.7093a587f4b749f18dacb0c003bd5ec2
Document Type :
article
Full Text :
https://doi.org/10.3390/bios14110517