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CRISPR-induced DNA reorganization for multiplexed nucleic acid detection

Authors :
Margot Karlikow
Evan Amalfitano
Xiaolong Yang
Jennifer Doucet
Abigail Chapman
Peivand Sadat Mousavi
Paige Homme
Polina Sutyrina
Winston Chan
Sofia Lemak
Alexander F. Yakunin
Adam G. Dolezal
Shana Kelley
Leonard J. Foster
Brock A. Harpur
Keith Pardee
Source :
Nature Communications, Vol 14, Iss 1, Pp 1-11 (2023)
Publication Year :
2023
Publisher :
Nature Portfolio, 2023.

Abstract

Nucleic acid sensing involving CRISPR technologies is powerful but has certain limitations, such as PAM sequence requirements and limited multiplexing. Here, authors report a CRISPR-based barcoding technology which enables multiple outputs from any target sequence, based on cis- and trans-cleavage.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
14
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.7000d2dde064ed7bf34db5ecc9e93ca
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-023-36874-6