Back to Search
Start Over
The importance of direct genetic testing for determining female carriers of the mutation in dystrophinopathies
- Source :
- Vojnosanitetski Pregled, Vol 80, Iss 3, Pp 201-207 (2023)
- Publication Year :
- 2023
- Publisher :
- Military Health Department, Ministry of Defance, Serbia, 2023.
-
Abstract
- Background/Aim. Duchenne muscular dystrophy (MD) and Becker MD are caused by mutations in the gene for dystrophin (DMD). They are X chromosome-linked recessive diseases where males are affected, and females are healthy carriers of the mutation in most cases. It is estimated that 2/3 of mothers of Duchenne MD probands are carriers, while 1/3 of probands have de novo mutations. The aim of the study was to confirm the carrier status of female members of the families of Duchenne MD/Becker MD probands using direct genetic testing methods. Methods. The study included 38 females from 31 families of Duchenne MD/Becker MD probands with deletion/duplication in the DMD gene. Moreover, 4 cases of prenatal diagnosis of Duchenne MD/Becker MD were included. The methods of polymerase chain reaction - PCR and the multiplex ligation-dependent probe amplification - MLPA were applied for detecting deletions, i.e., deletion/duplication mutations in the DMD gene. Results. In the total of 31 Duchenne MD/Becker MD probands, 87.1% of deletions and 12.9% of duplications of one or more exons in the DMD gene were detected. Of the 29 tested mothers, mutations were found in 17 of them (14 deletions and 3 duplications). Mutations were detected in 11 (57.9%) out of 19 mothers of probands with the Duchenne MD phenotype and 6 (60%) out of 10 mothers of Becker MD probands. Furthermore, 14 (56%) out of 25 mothers were carriers in probands with deletions, and 3 (75%) out of 4 mothers were carriers in probands with duplications. In the remaining 9 other female relatives of the patients, mutations were found in 4. In prenatal diagnosis, we identified a deletion in one male and one female fetus of one single mother who was confirmed as a carrier. Conclusion. The study showed that mothers were carriers in almost 60% of sporadic cases of Duchenne MD/Becker MD with deletions and duplications. In addition, the carrier frequency tended to be higher in mothers of the probands with duplications (75%) compared to mothers of probands with deletions (56%).
Details
- Language :
- English, Serbian
- ISSN :
- 00428450 and 24060720
- Volume :
- 80
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Vojnosanitetski Pregled
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.6fd6bf6b7de4f09b5aea2de747d5d71
- Document Type :
- article
- Full Text :
- https://doi.org/10.2298/VSP190208030M