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EVALUATION OF THE EXTRACELLULAR MATRIX OF INJURED SUPRASPINATUS IN RATS
- Source :
- Acta Ortopédica Brasileira, Vol 24, Iss 1, Pp 11-15 (2016)
- Publication Year :
- 2016
- Publisher :
- Sociedade Brasileira de Ortopedia e Traumatologia, 2016.
-
Abstract
- ABSTRACT Objective: To evaluate the evolution of injuries of the supraspinatus muscle by immunohistochemistry (IHC) and anatomopathological analysis in animal model (Wistar rats). Methods: Twenty-five Wistar rats were submitted to complete injury of the supraspinatus tendon, then subsequently sacrificed in groups of five animals at the following periods: immediately after the injury, 24h after the injury, 48h after, 30 days after and three months after the injury. All groups underwent histological and IHC analysis. Results: Regarding vascular proliferation and inflammatory infiltrate, we found a statistically significant difference between groups 1(control group) and 2 (24h after injury). IHC analysis showed that expression of vascular endothelial growth factor (VEGF) showed a statistically significant difference between groups 1 and 2, and collagen type 1 (Col-1) evaluation presented a statistically significant difference between groups 1 and 4. Conclusion: We observed changes in the extracellular matrix components compatible with remodeling and healing. Remodeling is more intense 24h after injury. However, VEGF and Col-1 are substantially increased at 24h and 30 days after the injury, respectively. Level of Evidence I, Experimental Study.
- Subjects :
- Collagen
Rats, Wistar
Extracellular Matrix
Medicine
Orthopedic surgery
RD701-811
Subjects
Details
- Language :
- English, Portuguese
- ISSN :
- 14137852
- Volume :
- 24
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Acta Ortopédica Brasileira
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.6fa437971e0348abbc63ad77636d9d42
- Document Type :
- article
- Full Text :
- https://doi.org/10.1590/1413-785220162401146706