ANALYSIS OF ASSOCIATIONS BETWEEN PROMOTER ALLELES OF TNFA, IL2. IL4 AND IL10, AND PARAMETERS OF IMMUNE SYSTEM IN HIV-INFECTED PATIENTS

Abstract. Establishment of basic processes leading to differences in immune response at genetic level occurs in the course of studying associations between certain polymorphic alleles, as well as evaluation of correlations between the haplotypes of cytokine genes, and specific protein production in vitro. The features of association between main parameters of humoral and cellular immunity, as well as levels of pro-inflammatory (TNFA и IL2) and anti-inflammatory (IL4 и IL10) cytokines with allelic variants of promoter regions of the mentioned genes were analyzed in HIV-infected patients, as compared with healthy Russian population of Caucasoid origin. In present work, we have performed genotyping, along with evaluation of humoral and cellular immunity, in 127 HIV-infected patients, and 52 donors served as a control group. To determine spontaneous and ConA-stimulated cytokine production in some patients, ELISA technique was used for assays with conditioned media from mononuclear cells. From the data obtained one may be inferred, that 308А TNFA, -330Т IL2, -592А IL10 alleles are more characteristic to the HIV-infected patients with decreased cellular pro-inflammatory component of immunity, and increased activity of B-cell immunity, in comparison with persons carrying other gene variants. In general, it should be stressed that the mentioned genes variants may determine general susceptibility for more severe course of disease, exerting some pleiotropic action upon some pathogenetically important parameters of HIV.