Back to Search Start Over

In vitro digestion and intestinal absorption of curcumin-loaded zein nanoparticles

Authors :
Negin Hashemi
Martin Krøyer Rasmussen
Emmanouil D. Tsochatzis
Milena Corredig
Source :
Journal of Functional Foods, Vol 110, Iss , Pp 105818- (2023)
Publication Year :
2023
Publisher :
Elsevier, 2023.

Abstract

Colloidal delivery systems improve the oral bioavailability of lipid-soluble bioactives, providing protection throughout the gastrointestinal tract. In this study, the fate of a model bioactive, curcumin, was followed during in vitro digestion and absorption, using state of the art INFOGEST in vitro models. Curcumin was loaded in zein nanoparticles, prepared by antisolvent precipitation, with or without sodium caseinate (Cur-Z-C and Cur-Z, respectively). The bioefficacy of the digesta were evaluated using Caco-2 cells. In addition, the absorption of curcumin was evaluated using a co-culture of Caco-2/HT29-MTX cells, which are mucus producing. Ultra-high-performance quadrupole time-of-flight mass spectrometry and cellular bioassays were utilized to assess the presence of bioaccessible material, and any residual curcumin after absorption. Zein nanoparticles were fully digested during gastrointestinal transit. A comparison between aqueous curcumin and digested Cur-Z and Cur-Z-C showed that Cur-Z-C decreased the proliferation of Caco-2 cells by ∼55%. This finding points out the importance of matrix effect on the efficacy of delivery systems.

Details

Language :
English
ISSN :
17564646
Volume :
110
Issue :
105818-
Database :
Directory of Open Access Journals
Journal :
Journal of Functional Foods
Publication Type :
Academic Journal
Accession number :
edsdoj.6ea9b029119437caa3798c821f13121
Document Type :
article
Full Text :
https://doi.org/10.1016/j.jff.2023.105818