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Exploring the Benefits of Metal Ions in Phage Cocktail for the Treatment of Methicillin-Resistant Staphylococcus aureus (MRSA) Infection
- Source :
- Infection and Drug Resistance, Vol Volume 15, Pp 2689-2702 (2022)
- Publication Year :
- 2022
- Publisher :
- Dove Medical Press, 2022.
-
Abstract
- Xinxin Li,1– 3 Yibao Chen,1– 3 Shuang Wang,1– 3 Xiaochao Duan,1– 3 Fenqiang Zhang,1– 3 Aizhen Guo,1– 3 Pan Tao,1– 3 Huanchun Chen,1– 3 Xiangmin Li,1– 3 Ping Qian1– 3 1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China; 2College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China; 3Key Laboratory of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, People’s Republic of ChinaCorrespondence: Ping Qian, Tel +86-27-87282608, Fax +86-27-87282608, Email qianp@mail.hzau.edu.cnBackground: Methicillin-resistant Staphylococcus aureus (MRSA) is an important zoonotic pathogen worldwide. Infections due to MRSA are associated with higher mortality rates compared with methicillin-susceptible S. aureus. Meanwhile, bacteriophages have been shown to overcome the emergence of MRSA.Methods: Phage PHB22a, PHB25a, PHB38a, and PHB40a were isolated. Here, we evaluated the ability of a phage cocktail containing phages PHB22a, PHB25a, PHB38a, and PHB40a against MRSA S-18 strain in vivo and in vitro. Phage whole-genome sequencing, host-range determination, lytic activity, and biofilm clearance experiments were performed in vitro. Galleria mellonella larvae and a mouse systemic infection model to evaluate the efficacy of phage therapy in vivo.Results: The phage cocktail exhibited enhanced antibacterial and anti-biofilm effects compared to the single phage. Phage cocktail contained with Ca2+/Zn2+ significantly reduced the number of viable bacteria (24-h or 48-h biofilm) by more than 0.81-log compared to the phage cocktail alone. Furthermore, we demonstrated that the addition of Ca2+ and Zn2+ phage cocktail could increase the survival rate of G. mellonella larvae infected with S. aureus by 10% compared with phage cocktail alone. This was further confirmed in the mouse model, which showed a 2.64-log reduction of host bacteria S-18, when Ca2+ and Zn2+ were included in the cocktail compared with the phage cocktail alone.Conclusion: Our results indicated that phage cocktail supplemented with Ca2+/Zn2+ could effectively remove bacteria in biofilms and mice tissues infected with S. aureus.Keywords: methicillin-resistant Staphylococcus aureus, phage cocktail, metal ions, biofilm, phage therapy
Details
- Language :
- English
- ISSN :
- 11786973
- Volume :
- ume 15
- Database :
- Directory of Open Access Journals
- Journal :
- Infection and Drug Resistance
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.6e9ca3e6972d4b0fbc719d9e7d1849c4
- Document Type :
- article